Anti-human FGF21 antibody
Developed by UnID Diagnostics Hong Kong (a wholly owned subsidiary of UnID)
Product introduction
Preparation method
Gst-human FGF21 protein expressed by E.coli was used as antigen to immunize the animals. The immunizing method was as follows:
1. The recombinant FGF21 antigen was emulsified with the same amount of Fredridge complete adjuvant, and the New Zealand white rabbit was injected subcutaneously into the back at multiple points.
2. Then every 1 to 2 weeks, the recombinant adiponectin antigen was emulsified with FRF's incomplete adjuvant, and the immunization was enhanced by repeated injection of the same method for 2 to 4 times;
3. Blood is extracted and purified after serum is obtained.
Purification method
The purification method is as follows:
1. The agarose gel column of cross-linked proteinA was used for affinity purification of antibodies in serum, and immunoglobulins, which were mainly IgG, were obtained.
2. The agarose gel column with cross-linked GST protein was used for secondary purification of the antibodies obtained in step 1. The antibodies that can bind to the GST antigen column were FGF21 non-specific antibodies, which were removed, and only the antibody flow fluid without gel column binding was collected.
3. The agarose gel column with cross-linked GST-human FGF21 protein was used to purify the antibody obtained in step 2 for three times. After the antibody was bound to the antigen gel column, the gel column was cleaned with a high-salt cleaning solution with PH6.0 containing 1M Nacl. After that, the eluent of ph5.0 is used to elute the last residual antibody on the gel column to obtain the final antibody product.
Gst-human FGF21 protein expressed by E.coli was used as antigen to immunize the animals. The immunizing method was as follows:
1. The recombinant FGF21 antigen was emulsified with the same amount of Fredridge complete adjuvant, and the New Zealand white rabbit was injected subcutaneously into the back at multiple points.
2. Then every 1 to 2 weeks, the recombinant adiponectin antigen was emulsified with FRF's incomplete adjuvant, and the immunization was enhanced by repeated injection of the same method for 2 to 4 times;
3. Blood is extracted and purified after serum is obtained.
Purification method
The purification method is as follows:
1. The agarose gel column of cross-linked proteinA was used for affinity purification of antibodies in serum, and immunoglobulins, which were mainly IgG, were obtained.
2. The agarose gel column with cross-linked GST protein was used for secondary purification of the antibodies obtained in step 1. The antibodies that can bind to the GST antigen column were FGF21 non-specific antibodies, which were removed, and only the antibody flow fluid without gel column binding was collected.
3. The agarose gel column with cross-linked GST-human FGF21 protein was used to purify the antibody obtained in step 2 for three times. After the antibody was bound to the antigen gel column, the gel column was cleaned with a high-salt cleaning solution with PH6.0 containing 1M Nacl. After that, the eluent of ph5.0 is used to elute the last residual antibody on the gel column to obtain the final antibody product.
